Drosophila: methods and protocols

Medientyp: Buch

Titel: Drosophila : methods and protocols

Enthält: Drosophila melanogaster and the development of biology in the 20th century / Alfonso Martinez Arias -- Getting started : an overview on raising and handling Drosophila / Hugo Stocker and Peter Gallant -- FlyBase : a database for the Drosophila research community / Rachel Drysdale and the FlyBase Consortium -- The use of P-element transposons to generate transgenic flies / André Bachmann and Elisabeth Knust -- The GAL4 system : a verstile system for the expression of genes / David A. Elliott and Andrea H. Brand -- P-element mutagenesis / Thomas Hummel and Christian Klämbt -- EMS screens : from mutagenesis to screening and mapping / Christian Bökel -- RNAi screening in cultured Drosophila cells / Sandra Steinbrink and Michael Boutros -- Methods for homologous recombination in Drosophila / Keith A. Maggert, Wei J. Gong, and Kent G. Golic -- Recombinases and their use in gene activation, gene inactivation, and transgenesis / Johannes Bischof and Konrad Basler -- Cuticle preparation of Drosophila embryos and larvae / Cyrille Alexandre -- Immunolabeling of embryos / H.-Arno J. Müller -- Imaging cellular and molecular dynamics in live embryos using fluorescent proteins / Matthieu Cavey and Thomas Lecuit -- Analysis of neuromuscular junctions : histology and in vivo imaging / Andreas Schmid and Stephan J. Sigrist -- Immunolabeling of imaginal discs / Thomas Klein -- Imaging Drosophila pupal wing morphogenesis / Anne-Kathrin Classen ... [et al.] -- Microscopic analysis of the adult Drosophila retina using semithin plastic section / Konstantin Gaengel and Marek Mlodzik -- Fluorescent in situ hybridization protocols in Drosophila embryos and tissues / Eric Lécuyer, Neela Parthasarathy, and Henry M. Krause -- Linear RNA amplification for the production of microarray hybridization probes / Ansgar Klebes and Thomas B. Kornberg -- An overview of the identification, detection, and functional analysis of Drosophila microRNAs / Nicholas S. Sokol -- Extraction and immunoblotting of proteins from embryos / Andreas Wodarz -- Purification of Drosophila protein complexes for mass spectrometry / Christoph Jüschke and Jürgen A. Knoblich -- Mass production of Drosophila embryos and chromatographic purification of native protein complexes / Natascha Kunert and Alexander Brehm -- Flow cytometric analysis of Drosophila cells / Aida Flor A. de la Cruz and Bruce A. Edgar -- Drosophila cell lines as model systems and as an experimental tool / Buzz Baum and Lucy Cherbas -- Appendix : phenotypic markers in Drosophila / Jennifer Childress and Georg Halder

Beteiligte: Dahmann, Christian [Hrsg.]

Erschienen: Totowa, N.J.: Humana Press, c2008

Umfang: XI, 437 S.; Ill., graph. Darst; 24 cm

Sprache: Englisch

ISBN: 9781588298171; 1588298175

RVK-Notation: WC 4460 : Molekulargenetische Methoden: DNS-Analyse, Klonierung, Mutagenese etc.
WC 6340 : Insekten
WQ 5555 : op - or

Schlagwörter: Drosophila > Molekularbiologie

Entstehung:

Anmerkungen: Includes bibliographical references and index. - Formerly CIP

Beschreibung: Drosophila melanogaster and the development of biology in the 20th century / Alfonso Martinez Arias -- Getting started : an overview on raising and handling Drosophila / Hugo Stocker and Peter Gallant -- FlyBase : a database for the Drosophila research community / Rachel Drysdale and the FlyBase Consortium -- The use of P-element transposons to generate transgenic flies / André Bachmann and Elisabeth Knust -- The GAL4 system : a verstile system for the expression of genes / David A. Elliott and Andrea H. Brand -- P-element mutagenesis / Thomas Hummel and Christian Klämbt -- EMS screens : from mutagenesis to screening and mapping / Christian Bökel -- RNAi screening in cultured Drosophila cells / Sandra Steinbrink and Michael Boutros -- Methods for homologous recombination in Drosophila / Keith A. Maggert, Wei J. Gong, and Kent G. Golic -- Recombinases and their use in gene activation, gene inactivation, and transgenesis / Johannes Bischof and Konrad Basler -- Cuticle preparation of Drosophila embryos and larvae / Cyrille Alexandre -- Immunolabeling of embryos / H.-Arno J. Müller -- Imaging cellular and molecular dynamics in live embryos using fluorescent proteins / Matthieu Cavey and Thomas Lecuit -- Analysis of neuromuscular junctions : histology and in vivo imaging / Andreas Schmid and Stephan J. Sigrist -- Immunolabeling of imaginal discs / Thomas Klein -- Imaging Drosophila pupal wing morphogenesis / Anne-Kathrin Classen ... [et al.] -- Microscopic analysis of the adult Drosophila retina using semithin plastic section / Konstantin Gaengel and Marek Mlodzik -- Fluorescent in situ hybridization protocols in Drosophila embryos and tissues / Eric Lécuyer, Neela Parthasarathy, and Henry M. Krause -- Linear RNA amplification for the production of microarray hybridization probes / Ansgar Klebes and Thomas B. Kornberg -- An overview of the identification, detection, and functional analysis of Drosophila microRNAs / Nicholas S. Sokol -- Extraction and immunoblotting of proteins from embryos / Andreas Wodarz -- Purification of Drosophila protein complexes for mass spectrometry / Christoph Jüschke and Jürgen A. Knoblich -- Mass production of Drosophila embryos and chromatographic purification of native protein complexes / Natascha Kunert and Alexander Brehm -- Flow cytometric analysis of Drosophila cells / Aida Flor A. de la Cruz and Bruce A. Edgar -- Drosophila cell lines as model systems and as an experimental tool / Buzz Baum and Lucy Cherbas -- Appendix : phenotypic markers in Drosophila / Jennifer Childress and Georg Halder

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